Short courses and workshops

 

Introduction to metabolomics workflow - by Coral Barbas and Serge Rudaz

The untargeted analysis of all the metabolites that change under a pathophysiological situation, or any wanted or unwanted action produced on a biological system, requires specific tools. In this short course, we will introduce the general concept of Metabolomics and it’s workflow. The main analytical platforms that are used, based on mass spectrometry will be presented, together with an introduction to the multivariate data analysis as well as Identification strategy of the instrumental signals to transform them into a name with a biological interpretation.

 

Facing the complexity of biopharmaceuticals characterisation - by Davy Guillarme and Yannis François

Protein biopharmaceuticals are macromolecules with a therapeutic effect, which are now more and more commonly used for the treatment of various diseases including cancer, diabetes, infection, inflammatory, and autoimmune disorders. However, protein biopharmaceuticals have a complexity far exceeding that of small molecule drugs. The goal of this short course is to introduce the general concepts about therapeutic proteins and provide some useful information on the analytical platforms that have to be used for their characterization. Among them, liquid chromatography (i.e. ion exchange, size exclusion, hydrophobic interaction, hydrophilic interaction, and reversed phase liquid chromatography) as well as capillary electrophoresis (i.e. capillary zone electrophoresis, gel capillary electrophoresis, capillary isoelectic focusing), coupled or not with mass spectrometry will be largely covered. Numerous applications to monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) will be used to illustrate the advantages and limitations of these diverse analytical approaches..

 

How to build an open-source capillary electrophoresis? - by Olivier Vorlet and Samuel Roth

In this 3h-workshop, a new generation open-source CE will be presented. This prototype, intended to be evolutionary and sustainable, was created by the University of Applied Sciences of Fribourg (Western Switzerland) in collaboration with the University of Geneva (Switzerland) and the Geneva University Hospitals (Switzerland). This CE device was conceived to help developing countries to fight falsified or sub-standard medicines, but it could also be useful for educational purposes. Interested users will get acquainted with the instrument, understand it and get trained on how to repair it. The new prototype includes a renewable energy source to make it independent from local supply, and an integrated software to simplify data treatment and reporting. Components are stardard and interchangeable, so they could be replaced by a local solution or easily available parts found on the Internet.

This workshop will be followed, during the whole symposium, by the complete assembly of the open source CE by two engineers of the University of Applied Sciences of Fribourg (Western Switzerland). A dedicated place in the exhibition area will be used to build this device from scratch. A video recording will be done during the process and a final demonstration will be performed at the end of the Symposium.